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Dushyant Patel Extractive Spectrophotometric Method

S.K.P.C.P.E.R., Kherva 75 M .Pharm. Thesis

y=0.0145x‐0.4744

=0.9954

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

30 40 50 60 70 80 90 100

Conce ntra ti on(µg/ml)

Absorbance

Figure 5.6 Calibration curve for GBP-BTB

TABLE NO: 5.1

REGRESSION ANALYSIS DATA AND SUMMARY OF VALIDATION

PARAMETERS FOR THE PROPOSED METHODS

Parameters BCG BTB

max

, nm 416 421

Beer Lambert’s law limits, µg/ml 10-120 40-90

Molar absorptivity* 1627.70 1123.72

Sandell’s sensitivity, µg/cm

/0.001

Absorbance unit*

0.1138 0.1750

Regression equation y=mx+c

y = 0.0065x + 0.0895 y = 0.0145x - 0.4744

Slope (m)

0.0065 0.0145

Intercept (c)

0.0895 -0.4744

Correlation coefficient (r

)

0.9966 0.9954

Limit of detection (LOD), µg/ml 2.90 10.86

Limit of quantification (LOQ), µg/ml 8.77 32.91

Repeatability (%RSD, n=6)

0.357 0.330

Precision (%RSD)

Interday (n = 3)

0.77-1.90 0.87-1.77

Intraday (n = 3)

0.14-1.08 0.31-1.63

* Mean of six determinations

5.3.2 Accuracy (% Recovery):

¾ The recovery experiments were performed by the standard addition method. The mean

recovery of GBP was 99.23 + 0.77 to 100.4 + 0.77 and 99.23+ 0.77 to 99.91 + 1.07 for

BCG in capsule and tablet dosage form, respectively (Table No. 5.2 and 5.3). The mean

recovery of GBP was 99.62 + 0.29 to 101.6 + 0.18 and 98.78 + 0.11 to 101.4 + 0.05 for

BTB in capsule and tablet dosage form, respectively (Table No. 5.2 and 5.3).The low

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